Coding

Part:BBa_K5507007:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


kana


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The kanamycin resistance system is well-established and commonly used in various bacterial species. Its typically results in minimal background growth, enhancing the efficiency of selection. In addition, since the Amp gene was included on the pGEM-T plasmid we used, we chose kana, another common resistance screening gene.


Source

The kana gene was amplified from the pET28a plasmid via PCR.

References